› 3bjb

X-ray diffraction
2.5Å resolution

Crystal structure of a TetR transcriptional regulator from Rhodococcus sp. RHA1

Released:
DOI: 10.2210/pdb3bjb/pdb
PDB entry 3bjb coloured by chain, front view.
PDB entry 3bjb coloured by chain, side view.
PDB entry 3bjb coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Tan K, Evdokimova E, Kudritska M, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 207 amino acids
Theoretical weight: 23.02 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q0S4E5 (Residues: 2-203; Coverage: 100%)
Gene name: RHA1_ro05814
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 21 x SO4
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 93.767Å b: 93.767Å c: 314.912Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.278
Expression system: Escherichia coli BL21

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