› 3bje

X-ray diffraction
1.44Å resolution

Crystal structure of Trypanosoma brucei nucleoside phosphorylase shows uridine phosphorylase activity

Released:
DOI: 10.2210/pdb3bje/pdb
PDB entry 3bje coloured by chain, front view.
PDB entry 3bje coloured by chain, side view.
PDB entry 3bje coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase.
Larson ET, Mudeppa DG, Gillespie JR, Mueller N, Napuli AJ, Arif JA, Ross J, Arakaki TL, Lauricella A, Detitta G, Luft J, Zucker F, Verlinde CL, Fan E, Van Voorhis WC, Buckner FS, Rathod PK, Hol WG, Merritt EA
J Mol Biol 396 1244-59 (2010)
PMID: 20070944

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside phosphorylase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 38 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57VZ2 (Residues: 1-341; Coverage: 100%)
Gene names: Tb08.29H22.830, Tb927.8.4430
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand R1P 2 x R1P
Ligand URA 2 x URA
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 63.015Å b: 95.387Å c: 63.483Å
α: 90° β: 105.91° γ: 90°
R-values:
R R work R free
0.156 0.155 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Pyrimidine metabolism in schistosomes: A comparison with other parasites and the search for potential chemotherapeutic targets.
El Kouni MH. (2017)
2 more

4 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
3 more