› 3bk1

X-ray diffraction
2.33Å resolution

Crystal Structure Analysis of RNase J

Released:
DOI: 10.2210/pdb3bk1/pdb
PDB entry 3bk1 coloured by chain, front view.
PDB entry 3bk1 coloured by chain, side view.
PDB entry 3bk1 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural insights into the dual activity of RNase J.
Li de la Sierra-Gallay I, Zig L, Jamalli A, Putzer H
Nat Struct Mol Biol 15 206-12 (2008)
PMID: 18204464

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease J Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 63.31 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72JJ7 (Residues: 20-573; Coverage: 97%)
Gene names: TT_C0775, rnj
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ZN 2 x ZN
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F222
Unit cell:
a: 97.83Å b: 117.62Å c: 230.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.22
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The many pathways of RNA degradation.
Houseley et al. (2009)
30 more

6 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
5 more