› 3bk2

X-ray diffraction
2.1Å resolution

Crystal Structure Analysis of the RNase J/UMP complex

Released:
DOI: 10.2210/pdb3bk2/pdb
PDB entry 3bk2 coloured by chain, front view.
PDB entry 3bk2 coloured by chain, side view.
PDB entry 3bk2 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural insights into the dual activity of RNase J.
Li de la Sierra-Gallay I, Zig L, Jamalli A, Putzer H
Nat Struct Mol Biol 15 206-12 (2008)
PMID: 18204464

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease J Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 63.31 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72JJ7 (Residues: 20-573; Coverage: 97%)
Gene names: TT_C0775, rnj
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand ZN 2 x ZN
Ligand U5P 1 x U5P
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: F222
Unit cell:
a: 98.08Å b: 118Å c: 228.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.217 0.236
Expression system: Escherichia coli

Quick links

Citations

33 review citations

The many pathways of RNA degradation.
Houseley et al. (2009)
32 more

3 mentions without citation

Loss of RNase J leads to multi-drug tolerance and accumulation of highly structured mRNA fragments in Mycobacterium tuberculosis.
Martini et al. (2022)
2 more