› 3bkf

X-ray diffraction
1.9Å resolution

Zinc-bound C-terminal Domain of NikR

Released:
DOI: 10.2210/pdb3bkf/pdb
Model geometry
Fit model/data
PDB entry 3bkf coloured by chain, front view.
PDB entry 3bkf coloured by chain, side view.
PDB entry 3bkf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of the metal specificity for nickel regulatory protein NikR.
Phillips CM, Schreiter ER, Guo Y, Wang SC, Zamble DB, Drennan CL
Biochemistry 47 1938-46 (2008)
PMID: 18193897

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-responsive regulator Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6Z6 (Residues: 48-133; Coverage: 65%)
Gene names: JW3446, b3481, nikR, yhhG
Sequence domains: NikR C terminal nickel binding domain
Structure domains: ACT-like. Chain A, domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6222
Unit cell:
a: 46.22Å b: 46.22Å c: 124.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.25 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Metalloproteins and metal sensing.
Waldron et al. (2009)
7 more

1 mention without citation

The Electronic Structure of the Metal Active Site Determines the Geometric Structure and Function of the Metalloregulator NikR.
Ha et al. (2019)