› 3bl9

X-ray diffraction
1.8Å resolution

Synthetic Gene Encoded DcpS bound to inhibitor DG157493

Released:
DOI: 10.2210/pdb3bl9/pdb
PDB entry 3bl9 coloured by chain, front view.
PDB entry 3bl9 coloured by chain, side view.
PDB entry 3bl9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DcpS as a therapeutic target for spinal muscular atrophy.
Singh J, Salcius M, Liu SW, Staker BL, Mishra R, Thurmond J, Michaud G, Mattoon DR, Printen J, Christensen J, Bjornsson JM, Pollok BA, Kiledjian M, Stewart L, Jarecki J, Gurney ME
ACS Chem Biol 3 711-22 (2008)
PMID: 18839960

Function and Biology Details

Reaction catalysed: 
A 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
m7GpppX diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 34.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96C86 (Residues: 38-337; Coverage: 89%)
Gene names: DCPS, DCS1, HINT5, HSPC015
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DD2 1 x DD2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 49.406Å b: 56.867Å c: 59.504Å
α: 62.35° β: 77.93° γ: 79.54°
R-values:
R R work R free
0.176 0.174 0.222
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Spinal muscular atrophy: mechanisms and therapeutic strategies.
Lorson et al. (2010)
23 more

4 mentions without citation

DcpS as a therapeutic target for spinal muscular atrophy.
Singh et al. (2008)
3 more