› 3blc

X-ray diffraction
2.5Å resolution

Crystal structure of the periplasmic domain of the Escherichia Coli YIDC

Released:
DOI: 10.2210/pdb3blc/pdb
PDB entry 3blc coloured by chain, front view.
PDB entry 3blc coloured by chain, side view.
PDB entry 3blc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the major periplasmic domain of the bacterial membrane protein assembly facilitator YidC.
Oliver DC, Paetzel M
J Biol Chem 283 5208-16 (2008)
PMID: 18093969

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-150564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane protein insertase YidC Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 35.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25714 (Residues: 26-340; Coverage: 58%)
Gene names: JW3683, b3705, yidC
Sequence domains: YidC periplasmic domain
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I4122
Unit cell:
a: 126.127Å b: 126.127Å c: 288.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
10 more

4 mentions without citation

The Sec System: Protein Export in Escherichia coli.
Crane et al. (2017)
3 more