› 3bmv

X-ray diffraction
1.6Å resolution

Cyclodextrin glycosyl transferase from Thermoanerobacterium thermosulfurigenes EM1 mutant S77P

Released:
DOI: 10.2210/pdb3bmv/pdb
PDB entry 3bmv coloured by chain, front view.
PDB entry 3bmv coloured by chain, side view.
PDB entry 3bmv coloured by chain, top view.
Source organism: Thermoanaerobacterium thermosulfurigenes
Primary publication:
Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution.
Kelly RM, Leemhuis H, Rozeboom HJ, van Oosterwijk N, Dijkstra BW, Dijkhuizen L
Biochem J 413 517-25 (2008)
PMID: 18422488

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 683 amino acids
Theoretical weight: 75.51 KDa
Source organism: Thermoanaerobacterium thermosulfurigenes
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P26827 (Residues: 28-710; Coverage: 100%)
Gene name: amyA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand SO4 5 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 73.625Å b: 96.016Å c: 114.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.163
Expression system: Bacillus subtilis

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Citations

10 review citations

Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.
Leemhuis et al. (2010)
9 more

2 mentions without citation

Core-controlled polymorphism in virus-like particles.
Sun et al. (2007)
1 more