› 3bnt

X-ray diffraction
2.3Å resolution

Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the Presence of [Co(NH3)6]Cl3 (A1555G mutant, Br-derivative)

Released:
DOI: 10.2210/pdb3bnt/pdb
PDB entry 3bnt coloured by chain, front view.
PDB entry 3bnt coloured by chain, side view.
PDB entry 3bnt coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates.
Kondo J, Westhof E
Nucleic Acids Res 36 2654-66 (2008)
PMID: 18346970

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196642 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
A site of human mitochondrial ribosome Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 7.09 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand NCO 1 x NCO
1 modified residue:
Ligand 5BU 1 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P64
Unit cell:
a: 74.865Å b: 74.865Å c: 22.537Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.255
Expression system: Not provided

Quick links

Citations

6 review citations

The future of aminoglycosides: the end or renaissance?
Houghton et al. (2010)
5 more

2 mentions without citation

The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates.
Kondo et al. (2008)
1 more