› 3bos

X-ray diffraction
1.75Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosomal replication initiator protein DnaA domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.52 KDa
Source organism: Shewanella amazonensis SB2B
Expression system: Escherichia coli
UniProt:
  • Canonical: A1S6W5 (Residues: 1-241; Coverage: 100%)
Gene name: Sama_1916
Sequence domains: Bacterial dnaA protein
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand MG 2 x MG
Ligand SCN 1 x SCN
Ligand CDP 2 x CDP
Ligand EDO 14 x EDO
Ligand NA 1 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 55.037Å b: 64.032Å c: 153.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.201
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulating DNA replication in bacteria.
Skarstad et al. (2013)
2 more

8 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
7 more