› 3boy

X-ray diffraction
1.7Å resolution

Crystal structure of the HutP antitermination complex bound to the HUT mRNA

Released:
DOI: 10.2210/pdb3boy/pdb
PDB entry 3boy coloured by chain, front view.
PDB entry 3boy coloured by chain, side view.
PDB entry 3boy coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kumarevel TS, Balasundaresan D, Jeyakanthan J, Shinkai A, Yokoyama S, Kumar PKR, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145537 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Hut operon positive regulatory protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 147 amino acids
Theoretical weight: 16.1 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P10943 (Residues: 2-148; Coverage: 99%)
Gene names: BSU39340, hutP
Sequence domains: HutP
Structure domains: Hut operon regulatory protein HutP
5'-R(*UP*UP*UP*AP*GP*UP*UP*UP*UP*UP*AP*GP*UP*UP*UP*UP*UP*AP*GP*UP*UP*U)-3' Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.88 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand HIS 3 x HIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 99.635Å b: 76.469Å c: 62.79Å
α: 90° β: 109.06° γ: 90°
R-values:
R R work R free
0.2 0.198 0.246
Expression systems:
  • Escherichia coli
  • Not provided

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