› 3bp3

X-ray diffraction
1.65Å resolution

Crystal structure of EIIB

Released:
DOI: 10.2210/pdb3bp3/pdb
PDB entry 3bp3 coloured by chain, front view.
PDB entry 3bp3 coloured by chain, side view.
PDB entry 3bp3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration.
Nam TW, Jung HI, An YJ, Park YH, Lee SH, Seok YJ, Cha SS
Proc Natl Acad Sci U S A 105 3751-6 (2008)
PMID: 18319344

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system glucose-specific EIICB component Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 8.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69786 (Residues: 396-477; Coverage: 17%)
Gene names: JW1087, b1101, glcA, ptsG, umg
Sequence domains: phosphotransferase system, EIIB
Structure domains: Glucose permease domain IIB

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 108.193Å b: 108.149Å c: 28.947Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.241
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.
Deutscher et al. (2014)
4 more

3 mentions without citation

Protein-protein docking benchmark version 4.0.
Hwang et al. (2010)
2 more