› 3bqg

X-ray diffraction
2Å resolution

Structure of the central domain (MsrA) of Neisseria meningitidis PilB (sulfenic acid form)

Released:
DOI: 10.2210/pdb3bqg/pdb
PDB entry 3bqg coloured by chain, front view.
PDB entry 3bqg coloured by chain, side view.
PDB entry 3bqg coloured by chain, top view.
Source organism: Neisseria meningitidis Z2491
Primary publication:
A structural analysis of the catalytic mechanism of methionine sulfoxide reductase A from Neisseria meningitidis.
Ranaivoson FM, Antoine M, Kauffmann B, Boschi-Muller S, Aubry A, Branlant G, Favier F
J Mol Biol 377 268-80 (2008)
PMID: 18255097

Function and Biology Details

Reactions catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (R)-S-oxide + thioredoxin
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide methionine sulfoxide reductase MsrA/MsrB Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.92 KDa
Source organism: Neisseria meningitidis Z2491
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JWM8 (Residues: 196-389; Coverage: 37%)
Gene names: NMA0290, msrAB, pilB
Sequence domains: Peptide methionine sulfoxide reductase
Structure domains: Peptide methionine sulphoxide reductase MsrA

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 44.56Å b: 54.61Å c: 64.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.24
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Oxidative stress, protein damage and repair in bacteria.
Ezraty et al. (2017)
4 more

1 mention without citation

Chemical approaches to detect and analyze protein sulfenic acids.
Furdui et al. (2014)