› 3brc

X-ray diffraction
1.6Å resolution

Crystal structure of a conserved protein of unknown function from Methanobacterium thermoautotrophicum

Released:
DOI: 10.2210/pdb3brc/pdb
PDB entry 3brc coloured by chain, front view.
PDB entry 3brc coloured by chain, side view.
PDB entry 3brc coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Entry authors: Zhang R, Evdokimova E, Kudritska M, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-127876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conserved protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.89 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O27213 (Residues: 1-156; Coverage: 100%)
Gene name: MTH_1144
Sequence domains: FeGP cofactor biosynthesis protein HcgB, guanylyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 106.894Å b: 48.916Å c: 63.868Å
α: 90° β: 118.54° γ: 90°
R-values:
R R work R free
0.186 0.183 0.237
Expression system: Escherichia coli BL21

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