› 3brq

X-ray diffraction
2Å resolution

Crystal structure of the Escherichia coli transcriptional repressor ascG

Released:
DOI: 10.2210/pdb3brq/pdb
PDB entry 3brq coloured by chain, front view.
PDB entry 3brq coloured by chain, side view.
PDB entry 3brq coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Singer AU, Kagan O, Evdokimova E, Osipiuk J, Joachimiak A, Edwards AM, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator AscG Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 32.91 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24242 (Residues: 43-336; Coverage: 88%)
Gene names: JW5434, ascG, b2714
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand FRU 1 x FRU
Ligand SO4 2 x SO4
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 62.229Å b: 65.446Å c: 130.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.249
Expression system: Escherichia coli BL21(DE3)

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