› 3brx

X-ray diffraction
2.5Å resolution

Crystal Structure of calcium-bound cotton annexin Gh1

Released:
DOI: 10.2210/pdb3brx/pdb
PDB entry 3brx coloured by chain, front view.
PDB entry 3brx coloured by chain, side view.
PDB entry 3brx coloured by chain, top view.
Source organism: Gossypium hirsutum
Primary publication:
The crystal structure of calcium-bound annexin Gh1 from Gossypium hirsutum and its implications for membrane binding mechanisms of plant annexins.
Hu NJ, Yusof AM, Winter A, Osman A, Reeve AK, Hofmann A
J Biol Chem 283 18314-22 (2008)
PMID: 18441010

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Annexin Gh1 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.15 KDa
Source organism: Gossypium hirsutum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P93157 (Residues: 1-315; Coverage: 100%)
Gene name: AnnGh1
Sequence domains: Annexin
Structure domains: Annexin

Ligands and Environments

2 bound ligands:
Ligand PO4 5 x PO4
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P321
Unit cell:
a: 132.663Å b: 132.663Å c: 61.338Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Annexins.
Laohavisit et al. (2011)
4 more

2 mentions without citation

Spatial arrangement of proteins in planar and curved membranes by PPM 3.0.
Lomize et al. (2022)
1 more