› 3bry

X-ray diffraction
3.2Å resolution

Crystal structure of the Ralstonia pickettii toluene transporter TbuX

Released:
DOI: 10.2210/pdb3bry/pdb
PDB entry 3bry coloured by chain, front view.
PDB entry 3bry coloured by chain, side view.
PDB entry 3bry coloured by chain, top view.
Source organism: Ralstonia pickettii
Primary publication:
Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation.
Hearn EM, Patel DR, van den Berg B
Proc Natl Acad Sci U S A 105 8601-6 (2008)
PMID: 18559855

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic hydrocarbon degradation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 46.28 KDa
Source organism: Ralstonia pickettii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RBW8 (Residues: 26-458; Coverage: 95%)
Gene names: D7S62_12410, tbuX
Sequence domains: Outer membrane protein transport protein (OMPP1/FadL/TodX)
Structure domains: Outer membrane protein transport protein (OMPP1/FadL/TodX)

Ligands and Environments

1 bound ligand:
Ligand C8E 2 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: F222
Unit cell:
a: 125.291Å b: 125.244Å c: 269.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.28
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Anaerobic catabolism of aromatic compounds: a genetic and genomic view.
Carmona et al. (2009)
7 more

12 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
11 more