› 3bs0

X-ray diffraction
2.6Å resolution

Crystal structure of the P. putida toluene transporter TodX

Released:
DOI: 10.2210/pdb3bs0/pdb
PDB entry 3bs0 coloured by chain, front view.
PDB entry 3bs0 coloured by chain, side view.
PDB entry 3bs0 coloured by chain, top view.
Source organism: Pseudomonas putida F1
Primary publication:
Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation.
Hearn EM, Patel DR, van den Berg B
Proc Natl Acad Sci U S A 105 8601-6 (2008)
PMID: 18559855

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane protein involved in aromatic hydrocarbon degradation Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 47.03 KDa
Source organism: Pseudomonas putida F1
Expression system: Escherichia coli
UniProt:
  • Canonical: A5W4F4 (Residues: 21-453; Coverage: 100%)
Gene name: Pput_2883
Sequence domains: Outer membrane protein transport protein (OMPP1/FadL/TodX)
Structure domains: Outer membrane protein transport protein (OMPP1/FadL/TodX)

Ligands and Environments

1 bound ligand:
Ligand C8E 4 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 38.098Å b: 41.415Å c: 141.064Å
α: 98.39° β: 90.01° γ: 90.06°
R-values:
R R work R free
0.253 0.253 0.291
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Anaerobic catabolism of aromatic compounds: a genetic and genomic view.
Carmona et al. (2009)
8 more

13 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
12 more