› 3bs3

X-ray diffraction
1.65Å resolution

Crystal structure of a putative DNA-binding protein from Bacteroides fragilis

Released:
DOI: 10.2210/pdb3bs3/pdb
PDB entry 3bs3 coloured by chain, front view.
PDB entry 3bs3 coloured by chain, side view.
PDB entry 3bs3 coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Cuff ME, Bigelow L, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.99 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5LGD2 (Residues: 1-73; Coverage: 100%)
Gene name: BF9343_1027
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 63.337Å b: 63.337Å c: 34.706Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.219
Expression system: Escherichia coli BL21(DE3)

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