› 3bs4

X-ray diffraction
1.6Å resolution

Crystal structure of uncharacterized protein PH0321 from Pyrococcus horikoshii in complex with an unknown peptide

Released:
DOI: 10.2210/pdb3bs4/pdb
PDB entry 3bs4 coloured by chain, front view.
PDB entry 3bs4 coloured by chain, side view.
PDB entry 3bs4 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Bonanno JB, Freeman J, Bain KT, Hu S, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129667 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
KaiC-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 30.54 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58059 (Residues: 3-251; Coverage: 98%)
Gene name: PH0321
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Unknown peptide Chain: B
Molecule details ›
Chain: B
Length: 3 amino acids
Theoretical weight: 392 Da
Source organism: Pyrococcus horikoshii OT3
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 97.241Å b: 71.121Å c: 49.581Å
α: 90° β: 93.01° γ: 90°
R-values:
R R work R free
0.185 0.184 0.208
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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