› 3bsm

X-ray diffraction
2.2Å resolution

Crystal structure of D-mannonate dehydratase from Chromohalobacter salexigens

Released:
DOI: 10.2210/pdb3bsm/pdb
PDB entry 3bsm coloured by chain, front view.
PDB entry 3bsm coloured by chain, side view.
PDB entry 3bsm coloured by chain, top view.
Source organism: Chromohalobacter salexigens DSM 3043
Entry authors: Fedorov AA, Fedorov EV, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reactions catalysed: 
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-173010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase family member ManD Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 413 amino acids
Theoretical weight: 46.48 KDa
Source organism: Chromohalobacter salexigens DSM 3043
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1QT89 (Residues: 2-403; Coverage: 100%)
Gene names: Csal_2974, manD
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 110.125Å b: 163.07Å c: 84.553Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.268
Expression system: Escherichia coli

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