› 3bsw

X-ray diffraction
1.77Å resolution

PglD-citrate complex, from Campylobacter jejuni NCTC 11168

Released:
DOI: 10.2210/pdb3bsw/pdb
PDB entry 3bsw coloured by chain, front view.
PDB entry 3bsw coloured by chain, side view.
PDB entry 3bsw coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Crystal structure and catalytic mechanism of PglD from Campylobacter jejuni.
Olivier NB, Imperiali B
J Biol Chem 283 27937-27946 (2008)
PMID: 18667421

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + UDP-N-acetylbacillosamine = CoA + UDP-N,N'-diacetylbacillosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylbacillosamine N-acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 21.39 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P9D1 (Residues: 1-195; Coverage: 100%)
Gene names: Cj1123c, pglD
Sequence domains: PglD N-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P63
Unit cell:
a: 86.347Å b: 86.347Å c: 65.538Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.199
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Protein glycosylation in bacteria: sweeter than ever.
Nothaft et al. (2010)
3 more

4 mentions without citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
3 more