› 3bu2

X-ray diffraction
2.7Å resolution

Crystal structure of a tRNA-binding protein from Staphylococcus saprophyticus subsp. saprophyticus. Northeast Structural Genomics Consortium target SyR77

Released:
DOI: 10.2210/pdb3bu2/pdb
PDB entry 3bu2 coloured by chain, front view.
PDB entry 3bu2 coloured by chain, side view.
PDB entry 3bu2 coloured by chain, top view.
Source organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292
Entry authors: Seetharaman J, Su M, Forouhar F, Wang D, Fang Y, Cunningham K, Ma L-C, Xia R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 21.68 KDa
Source organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292
Expression system: Escherichia coli
UniProt:
  • Canonical: Q49YH3 (Residues: 1-199; Coverage: 100%)
Gene name: SSP1021
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 75.902Å b: 79.795Å c: 139.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.273
Expression system: Escherichia coli

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