› 3buu

X-ray diffraction
1.2Å resolution

Crystal structure of LolA superfamily protein NE2245 from Nitrosomonas europaea

Released:
DOI: 10.2210/pdb3buu/pdb
PDB entry 3buu coloured by chain, front view.
PDB entry 3buu coloured by chain, side view.
PDB entry 3buu coloured by chain, top view.
Source organism: Nitrosomonas europaea ATCC 19718
Entry authors: Chang C, Wu R, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane lipoprotein-sorting protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.54 KDa
Source organism: Nitrosomonas europaea ATCC 19718
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82SR2 (Residues: 32-257; Coverage: 95%)
Gene name: NE2245
Sequence domains: Outer membrane lipoprotein-sorting protein
Structure domains: Lipoprotein localisation LolA/LolB/LppX

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 35.019Å b: 59.116Å c: 89.676Å
α: 90° β: 90.72° γ: 90°
R-values:
R R work R free
0.16 0.158 0.192
Expression system: Escherichia coli

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