› 3bvq

X-ray diffraction
2.8Å resolution

Crystal Structure of Apo NotI Restriction Endonuclease

Released:
DOI: 10.2210/pdb3bvq/pdb
PDB entry 3bvq coloured by chain, front view.
PDB entry 3bvq coloured by chain, side view.
PDB entry 3bvq coloured by chain, top view.
Source organism: Nocardia otitidiscaviarum
Primary publication:
Structures of the rare-cutting restriction endonuclease NotI reveal a unique metal binding fold involved in DNA binding.
Lambert AR, Sussman D, Shen B, Maunus R, Nix J, Samuelson J, Xu SY, Stoddard BL
Structure 16 558-69 (2008)
PMID: 18400177

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NotI restriction endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 42.75 KDa
Source organism: Nocardia otitidiscaviarum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2I6W2 (Residues: 1-383; Coverage: 100%)
Gene names: FOH10_30205, notIR

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 74.893Å b: 74.893Å c: 274.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.262 0.335
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Type II restriction endonucleases--a historical perspective and more.
Pingoud et al. (2014)
18 other articles

2 mentions without citation

Structures of the rare-cutting restriction endonuclease NotI reveal a unique metal binding fold involved in DNA binding.
Lambert et al. (2008)
1 more