› 3bw1

X-ray diffraction
2.5Å resolution

Crystal structure of homomeric yeast Lsm3 exhibiting novel octameric ring organisation

Released:
DOI: 10.2210/pdb3bw1/pdb
PDB entry 3bw1 coloured by chain, front view.
PDB entry 3bw1 coloured by chain, side view.
PDB entry 3bw1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of Lsm3 octamer from Saccharomyces cerevisiae: implications for Lsm ring organisation and recruitment.
Naidoo N, Harrop SJ, Sobti M, Haynes PA, Szymczyna BR, Williamson JR, Curmi PM, Mabbutt BC
J Mol Biol 377 1357-71 (2008)
PMID: 18329667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U6 snRNA-associated Sm-like protein LSm3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P57743 (Residues: 1-89; Coverage: 100%)
Gene names: LSM3, SMX4, USS2, YLR438C-A
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

2 bound ligands:
Ligand MPD 4 x MPD
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I422
Unit cell:
a: 83.743Å b: 83.743Å c: 141.931Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.243
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The bacterial protein Hfq: much more than a mere RNA-binding factor.
Sobrero et al. (2012)
3 more

6 mentions without citation

Lsm proteins and Hfq: Life at the 3' end.
Wilusz et al. (2013)
5 more