› 3bwb

X-ray diffraction
2.5Å resolution

Crystal structure of the apo form of spermidine synthase from Trypanosoma cruzi at 2.5 A resolution

Released:
DOI: 10.2210/pdb3bwb/pdb
PDB entry 3bwb coloured by chain, front view.
PDB entry 3bwb coloured by chain, side view.
PDB entry 3bwb coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Entry authors: Bosch J, Hol WGJ, Structural Genomics of Pathogenic Protozoa Consortium (SGPP)

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.53 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DA73 (Residues: 1-296; Coverage: 100%)
Gene name: Tc00.1047053510339.50
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 43.669Å b: 96.996Å c: 69.649Å
α: 90° β: 106.33° γ: 90°
R-values:
R R work R free
0.201 0.198 0.248
Expression system: Escherichia coli

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