› 3bwc

X-ray diffraction
2.3Å resolution

Crystal structure of spermidine synthase from Trypanosoma cruzi in complex with SAM at 2.3 A resolution

Released:
DOI: 10.2210/pdb3bwc/pdb
PDB entry 3bwc coloured by chain, front view.
PDB entry 3bwc coloured by chain, side view.
PDB entry 3bwc coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Entry authors: Bosch J, Arakaki TL, Le Trong I, Merritt EA, Hol WGJ, Structural Genomics of Pathogenic Protozoa Consortium (SGPP)

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.53 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DA73 (Residues: 1-296; Coverage: 100%)
Gene name: Tc00.1047053510339.50
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SAM 2 x SAM
Ligand BME 2 x BME
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 44.08Å b: 99.943Å c: 69.991Å
α: 90° β: 106.26° γ: 90°
R-values:
R R work R free
0.206 0.203 0.259
Expression system: Escherichia coli

Quick links