› 3bwl

X-ray diffraction
1.73Å resolution

Crystal structure of PAS domain of HTR-like protein from Haloarcula marismortui

Released:
DOI: 10.2210/pdb3bwl/pdb
PDB entry 3bwl coloured by chain, front view.
PDB entry 3bwl coloured by chain, side view.
PDB entry 3bwl coloured by chain, top view.
Source organism: Haloarcula marismortui ATCC 43049
Entry authors: Osipiuk J, Zhou M, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 126 amino acids
Theoretical weight: 14.84 KDa
Source organism: Haloarcula marismortui ATCC 43049
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5V5P7 (Residues: 387-509; Coverage: 17%)
Gene names: E6P14_19500, htlD, rrnAC0075
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand I3A 4 x I3A
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 66.811Å b: 68.533Å c: 105.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.197
Expression system: Escherichia coli BL21(DE3)

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