› 3bwt

X-ray diffraction
2.69Å resolution

Crystal structure of the RNA binding domain of Puf4 from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3bwt/pdb
PDB entry 3bwt coloured by chain, front view.
PDB entry 3bwt coloured by chain, side view.
PDB entry 3bwt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p.
Miller MT, Higgin JJ, Hall TM
Nat Struct Mol Biol 15 397-402 (2008)
PMID: 18327269

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pumilio homology domain family member 4 Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 37.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25339 (Residues: 554-886; Coverage: 38%)
Gene names: PUF4, YGL014W, YGL023
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 54.188Å b: 137.657Å c: 137.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.27
Expression system: Escherichia coli

Quick links

Citations

13 review citations

PUF proteins: repression, activation and mRNA localization.
Quenault et al. (2011)
12 more

3 mentions without citation

Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p.
Miller et al. (2008)
2 more