› 3bxz

X-ray diffraction
3Å resolution

Crystal structure of the isolated DEAD motor domains from Escherichia coli SecA

Released:
DOI: 10.2210/pdb3bxz/pdb
PDB entry 3bxz coloured by chain, front view.
PDB entry 3bxz coloured by chain, side view.
PDB entry 3bxz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Analysis of the isolated SecA DEAD motor suggests a mechanism for chemical-mechanical coupling.
Nithianantham S, Shilton BH
J Mol Biol 383 380-9 (2008)
PMID: 18761349

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA Chains: A, B
Molecule details ›
Chains: A, B
Length: 471 amino acids
Theoretical weight: 52.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P10408 (Residues: 6-609; Coverage: 52%)
Gene names: JW0096, azi, b0098, pea, prlD, secA
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand SPD 2 x SPD
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 177.823Å b: 71.361Å c: 119.536Å
α: 90° β: 128.82° γ: 90°
R-values:
R R work R free
0.245 0.245 0.305
Expression system: Escherichia coli

Quick links

Citations

4 review citations

SecA: a tale of two protomers.
Sardis et al. (2010)
3 more

1 mention without citation

SecA: a potential antimicrobial target.
Chaudhary et al. (2015)