› 3byy

X-ray diffraction
2.2Å resolution

Manipulating the coupled folding and binding process drives affinity maturation in a protein-protein complex

Released:
DOI: 10.2210/pdb3byy/pdb
PDB entry 3byy coloured by chain, front view.
PDB entry 3byy coloured by chain, side view.
PDB entry 3byy coloured by chain, top view.
Source organisms:
Entry authors: Cho S, Eric JS

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209435 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T cell receptor beta chain 8.2 Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 11.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Enterotoxin type C-3 Chain: B
Molecule details ›
Chain: B
Length: 237 amino acids
Theoretical weight: 27.65 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0L5 (Residues: 28-266; Coverage: 99%)
Gene name: entC3
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P65
Unit cell:
a: 96.69Å b: 96.69Å c: 91.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.233
Expression system: Escherichia coli

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