› 3bzs

X-ray diffraction
1.48Å resolution

Crystal structure of EscU C-terminal domain with N262D mutation, Space group P 21 21 21

Released:
DOI: 10.2210/pdb3bzs/pdb
PDB entry 3bzs coloured by chain, front view.
PDB entry 3bzs coloured by chain, side view.
PDB entry 3bzs coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.
Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC
Nature 453 124-7 (2008)
PMID: 18451864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EscU protein Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 215-345; Coverage: 38%)
Gene names: BG944_004520, C0P57_005208, CTR35_004860, EAN77_23705, EAX79_25000, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, escU
Sequence domains: FlhB HrpN YscU SpaS Family
Structure domains: secretion proteins EscU

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 31.371Å b: 48.349Å c: 60.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

29 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
28 more