3c0t

X-ray diffraction
2.4Å resolution

Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-126952 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 18 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 24.06 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O14198 (Residues: 1-207; Coverage: 100%)
Gene names: SPAC5D6.05, med18, pmc6, sep11
Sequence domains: Med18 protein
Structure domains: Hypothetical Protein Pfu-838710-001
Mediator of RNA polymerase II transcription subunit 8 Chain: B
Molecule details ›
Chain: B
Length: 33 amino acids
Theoretical weight: 3.86 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O94646 (Residues: 180-200; Coverage: 11%)
Gene names: SPBC21.04, med8, sep15

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 111.016Å b: 111.016Å c: 68.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.231
Expression system: Escherichia coli