› 3c1g

X-ray diffraction
2.3Å resolution

Substrate binding, deprotonation and selectivity at the periplasmic entrance of the E. coli ammonia channel AmtB

Released:
DOI: 10.2210/pdb3c1g/pdb
PDB entry 3c1g coloured by chain, front view.
PDB entry 3c1g coloured by chain, side view.
PDB entry 3c1g coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Substrate binding, deprotonation, and selectivity at the periplasmic entrance of the Escherichia coli ammonia channel AmtB.
Javelle A, Lupo D, Ripoche P, Fulford T, Merrick M, Winkler FK
Proc Natl Acad Sci U S A 105 5040-5 (2008)
PMID: 18362341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter AmtB Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 44.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P63
Unit cell:
a: 109.329Å b: 109.329Å c: 84.821Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.185 0.242
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Nitrogen assimilation in Escherichia coli: putting molecular data into a systems perspective.
van Heeswijk et al. (2013)
11 more

1 mention without citation

Substrate binding, deprotonation, and selectivity at the periplasmic entrance of the Escherichia coli ammonia channel AmtB.
Javelle et al. (2008)