› 3c1o

X-ray diffraction
1.8Å resolution

The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct lineages

Released:
DOI: 10.2210/pdb3c1o/pdb
PDB entry 3c1o coloured by chain, front view.
PDB entry 3c1o coloured by chain, side view.
PDB entry 3c1o coloured by chain, top view.
Source organism: Clarkia breweri
Primary publication:
The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages.
Koeduka T, Louie GV, Orlova I, Kish CM, Ibdah M, Wilkerson CG, Bowman ME, Baiga TJ, Noel JP, Dudareva N, Pichersky E
Plant J 54 362-74 (2008)
PMID: 18208524

Function and Biology Details

Reaction catalysed: 
Eugenol + a carboxylate + NADP(+) = a coniferyl ester + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-112088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eugenol synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 36.34 KDa
Source organism: Clarkia breweri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VWT0 (Residues: 1-318; Coverage: 100%)
Gene name: EGS1
Sequence domains: NmrA-like family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAP 1 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 67.33Å b: 87.381Å c: 51.545Å
α: 90° β: 101.33° γ: 90°
R-values:
R R work R free
0.186 0.186 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Biosynthesis, function and metabolic engineering of plant volatile organic compounds.
Dudareva et al. (2013)
7 more

1 mention without citation

Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis.
Qiang et al. (2024)