› 3c1r

X-ray diffraction
2Å resolution

Crystal structure of oxidized GRX1

Released:
DOI: 10.2210/pdb3c1r/pdb
PDB entry 3c1r coloured by chain, front view.
PDB entry 3c1r coloured by chain, side view.
PDB entry 3c1r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Glutathionylation-triggered conformational changes of glutaredoxin Grx1 from the yeast Saccharomyces cerevisiae.
Yu J, Zhang NN, Yin PD, Cui PX, Zhou CZ
Proteins 72 1077-83 (2008)
PMID: 18473363

Function and Biology Details

Reactions catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaredoxin-1 Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25373 (Residues: 1-110; Coverage: 100%)
Gene names: GRX1, YCL035C, YCL35C
Sequence domains: Glutaredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 28.21Å b: 49.631Å c: 39.701Å
α: 90° β: 94.95° γ: 90°
R-values:
R R work R free
0.193 0.193 0.205
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
4 more

7 mentions without citation

Comparison of structures among Saccharomyces cerevisiae Grxs proteins.
Abdalla et al. (2018)
6 more