› 3c1y

X-ray diffraction
2.1Å resolution

Structure of bacterial DNA damage sensor protein with co-purified and co-crystallized ligand

Released:
DOI: 10.2210/pdb3c1y/pdb
PDB entry 3c1y coloured by chain, front view.
PDB entry 3c1y coloured by chain, side view.
PDB entry 3c1y coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.
Witte G, Hartung S, Büttner K, Hopfner KP
Mol Cell 30 167-78 (2008)
PMID: 18439896

Function and Biology Details

Reaction catalysed: 
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA integrity scanning protein DisA Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 42.76 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WY43 (Residues: 1-357; Coverage: 100%)
Gene names: TM_0200, disA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 2BA 1 x 2BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA, ESRF BEAMLINE ID29
Spacegroup: P4212
Unit cell:
a: 108.329Å b: 108.329Å c: 166.415Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.257
Expression system: Escherichia coli

Quick links

Citations

64 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
63 more

8 mentions without citation

Making and Breaking of an Essential Poison: the Cyclases and Phosphodiesterases That Produce and Degrade the Essential Second Messenger Cyclic di-AMP in Bacteria.
Commichau et al. (2019)
7 more