› 3c3e

X-ray diffraction
3Å resolution

Crystal structure of 2-phospho-(S)-lactate transferase from Methanosarcina mazei in complex with Fo and GDP. Northeast Structural Genomics Consortium target MaR46

Released:
DOI: 10.2210/pdb3c3e/pdb
PDB entry 3c3e coloured by chain, front view.
PDB entry 3c3e coloured by chain, side view.
PDB entry 3c3e coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Primary publication:
Molecular insights into the biosynthesis of the F420 coenzyme.
Forouhar F, Abashidze M, Xu H, Grochowski LL, Seetharaman J, Hussain M, Kuzin A, Chen Y, Zhou W, Xiao R, Acton TB, Montelione GT, Galinier A, White RH, Tong L
J Biol Chem 283 11832-40 (2008)
PMID: 18252724

Function and Biology Details

Reaction catalysed: 
(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + factor 420-0
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-phospho-L-lactate transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 34.84 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PVT6 (Residues: 1-303; Coverage: 100%)
Gene names: MM_1874, cofD
Sequence domains: 2-phospho-L-lactate transferase CofD
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FO1 4 x FO1
Ligand GDP 4 x GDP
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P32
Unit cell:
a: 186.544Å b: 186.544Å c: 67.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.237
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
2 more

2 mentions without citation

Mutations in genes for the F420 biosynthetic pathway and a nitroreductase enzyme are the primary resistance determinants in spontaneous in vitro-selected PA-824-resistant mutants of Mycobacterium tuberculosis.
Haver et al. (2015)
1 more