› 3c4a

X-ray diffraction
2.3Å resolution

Crystal structure of vioD hydroxylase in complex with FAD from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvR158

Released:
DOI: 10.2210/pdb3c4a/pdb
PDB entry 3c4a coloured by chain, front view.
PDB entry 3c4a coloured by chain, side view.
PDB entry 3c4a coloured by chain, top view.
Source organism: Chromobacterium violaceum ATCC 12472
Entry authors: Forouhar F, Neely H, Seetharaman J, Janjua H, Xiao R, Maglaqui M, Wang H, Baran MC, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Protodeoxyviolaceinate + NAD(P)H + O(2) = protoviolaceinate + NAD(P)(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protodeoxyviolaceinate monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 43.17 KDa
Source organism: Chromobacterium violaceum ATCC 12472
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S3U8 (Residues: 1-373; Coverage: 100%)
Gene names: CV_3271, vioD
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21212
Unit cell:
a: 89.179Å b: 97.725Å c: 40.605Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.262
Expression system: Escherichia coli

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