› 3c4h

X-ray diffraction
2.1Å resolution

Human poly(ADP-ribose) polymerase 3, catalytic fragment in complex with an inhibitor DR2313

Released:
DOI: 10.2210/pdb3c4h/pdb
PDB entry 3c4h coloured by chain, front view.
PDB entry 3c4h coloured by chain, side view.
PDB entry 3c4h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for inhibitor specificity in human poly(ADP-ribose) polymerase-3.
Lehtiö L, Jemth AS, Collins R, Loseva O, Johansson A, Markova N, Hammarström M, Flores A, Holmberg-Schiavone L, Weigelt J, Helleday T, Schüler H, Karlberg T
J Med Chem 52 3108-11 (2009)
PMID: 19354255

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein mono-ADP-ribosyltransferase PARP3 Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 39.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y6F1 (Residues: 178-532; Coverage: 67%)
Gene names: ADPRT3, ADPRTL3, PARP3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DRL 1 x DRL
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 54.91Å b: 57.33Å c: 56.26Å
α: 90° β: 112.36° γ: 90°
R-values:
R R work R free
0.178 0.174 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

PARP inhibition: PARP1 and beyond.
Rouleau et al. (2010)
18 more

6 mentions without citation

PARP-3 is a mono-ADP-ribosylase that activates PARP-1 in the absence of DNA.
Loseva et al. (2010)
5 more