› 3c4z

X-ray diffraction
1.84Å resolution

Crystal structure of G protein coupled receptor kinase 1 bound to ADP and magnesium chloride at 1.84A

Released:
DOI: 10.2210/pdb3c4z/pdb
PDB entry 3c4z coloured by chain, front view.
PDB entry 3c4z coloured by chain, side view.
PDB entry 3c4z coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation.
Singh P, Wang B, Maeda T, Palczewski K, Tesmer JJ
J Biol Chem 283 14053-62 (2008)
PMID: 18339619

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin kinase GRK1 Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 61.43 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28327 (Residues: 1-535; Coverage: 95%)
Gene names: GRK1, RHOK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CL 2 x CL
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 65.54Å b: 82.947Å c: 107.144Å
α: 90° β: 93.01° γ: 90°
R-values:
R R work R free
0.193 0.193 not available
Expression system: Trichoplusia ni

Quick links

Citations

17 review citations

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.
Gurevich et al. (2012)
16 more

7 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
6 more