› 3c5i

X-ray diffraction
2.2Å resolution

Crystal structure of Plasmodium knowlesi choline kinase, PKH_134520

Released:
DOI: 10.2210/pdb3c5i/pdb
PDB entry 3c5i coloured by chain, front view.
PDB entry 3c5i coloured by chain, side view.
PDB entry 3c5i coloured by chain, top view.
Source organism: Plasmodium knowlesi
Entry authors: Wernimont AK, Hills T, Lew J, Wasney G, Senesterra G, Kozieradzki I, Cossar D, Vedadi M, Schapira M, Bochkarev A, Arrowsmith CH, Bountra C, Weigelt J, Edwards AM, Hui R, Artz JD, Xiao T, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112090 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Choline kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 369 amino acids
Theoretical weight: 43.84 KDa
Source organism: Plasmodium knowlesi
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWT1 (Residues: 2-369; Coverage: 100%)
Gene name: PKH_134520
Sequence domains: Choline/ethanolamine kinase
Structure domains:
Cleaved fragment of N-terminal expression tag Chain: E
Molecule details ›
Chain: E
Length: 6 amino acids
Theoretical weight: 813 Da
Source organism: Plasmodium knowlesi
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand CA 4 x CA
Ligand CHT 2 x CHT
Ligand GOL 10 x GOL
Ligand UNL 3 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 112.985Å b: 71.993Å c: 116.393Å
α: 90° β: 97.72° γ: 90°
R-values:
R R work R free
0.215 0.212 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links