› 3c71

X-ray diffraction
1.9Å resolution

Structure of a ResA variant with a DsbA-like active site motif (CPHC)

Released:
DOI: 10.2210/pdb3c71/pdb
PDB entry 3c71 coloured by chain, front view.
PDB entry 3c71 coloured by chain, side view.
PDB entry 3c71 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Effects of substitutions in the CXXC active-site motif of the extracytoplasmic thioredoxin ResA.
Lewin A, Crow A, Hodson CT, Hederstedt L, Le Brun NE
Biochem J 414 81-91 (2008)
PMID: 18422485

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-disulfide oxidoreductase ResA Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 15.95 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P35160 (Residues: 37-179; Coverage: 80%)
Gene names: BSU23150, resA, ypxA
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P65
Unit cell:
a: 36.975Å b: 36.975Å c: 177.665Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.164 0.211
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure, function, and mechanism of thioredoxin proteins.
Collet et al. (2010)
4 more