› 3c72

X-ray diffraction
2.3Å resolution

Engineered RabGGTase in complex with a peptidomimetic inhibitor

Released:
DOI: 10.2210/pdb3c72/pdb
PDB entry 3c72 coloured by chain, front view.
PDB entry 3c72 coloured by chain, side view.
PDB entry 3c72 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Development of selective RabGGTase inhibitors and crystal structure of a RabGGTase-inhibitor complex.
Guo Z, Wu YW, Tan KT, Bon RS, Guiu-Rozas E, Delon C, Nguyen TU, Wetzel S, Arndt S, Goody RS, Blankenfeldt W, Alexandrov K, Waldmann H
Angew Chem Int Ed Engl 47 3747-50 (2008)
PMID: 18399557

Function and Biology Details

Reaction catalysed: 
Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170665 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Geranylgeranyl transferase type-2 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 38.69 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08602 (Residues: 1-236, 353-441; Coverage: 57%)
Gene names: Ggta, Rabggta
Sequence domains: Protein prenyltransferase alpha subunit repeat
Structure domains: Protein prenylyltransferase
Geranylgeranyl transferase type-2 subunit beta Chain: B
Molecule details ›
Chain: B
Length: 331 amino acids
Theoretical weight: 36.89 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08603 (Residues: 1-331; Coverage: 100%)
Gene names: Ggtb, Rabggtb
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains: Glycosyltransferase

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
Ligand CX1 1 x CX1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 66.192Å b: 91.671Å c: 115.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.256
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Chemical tools for understanding protein lipidation in eukaryotes.
Charron et al. (2009)
2 more

3 mentions without citation

Protein Prenyltransferases and Their Inhibitors: Structural and Functional Characterization.
Marchwicka et al. (2022)
2 more