› 3c8t

X-ray diffraction
2.2Å resolution

Crystal structure of fumarate lyase from Mesorhizobium sp. BNC1

Released:
DOI: 10.2210/pdb3c8t/pdb
PDB entry 3c8t coloured by chain, front view.
PDB entry 3c8t coloured by chain, side view.
PDB entry 3c8t coloured by chain, top view.
Source organism: Chelativorans sp. BNC1
Entry authors: Bonanno JB, Freeman J, Bain KT, Chang S, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 49.37 KDa
Source organism: Chelativorans sp. BNC1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q11C84 (Residues: 17-456; Coverage: 97%)
Gene name: Meso_3622
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P42212
Unit cell:
a: 107.006Å b: 107.006Å c: 74.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.262
Expression system: Escherichia coli BL21(DE3)

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