› 3c94

X-ray diffraction
2.7Å resolution

ExoI/SSB-Ct complex

Released:
DOI: 10.2210/pdb3c94/pdb
Model geometry
Fit model/data
PDB entry 3c94 coloured by chain, front view.
PDB entry 3c94 coloured by chain, side view.
PDB entry 3c94 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Escherichia coli single-stranded DNA-binding protein stimulation of exonuclease I.
Lu D, Keck JL
Proc Natl Acad Sci U S A 105 9169-74 (2008)
PMID: 18591666

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283864 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exodeoxyribonuclease I Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04995 (Residues: 1-475; Coverage: 100%)
Gene names: JW1993, b2011, cpeA, sbcB, xonA
Sequence domains:
Structure domains:
Single-stranded DNA-binding protein Chains: B, C
Molecule details ›
Chains: B, C
Length: 10 amino acids
Theoretical weight: 1.3 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0AGE0 (Residues: 170-178; Coverage: 5%)
Gene names: JW4020, b4059, exrB, lexC, ssb

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 67.151Å b: 92.191Å c: 91.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

The genetic transformation machinery: composition, localization, and mechanism.
Claverys et al. (2009)
6 more

9 mentions without citation

Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp.
Robinson et al. (2010)
8 more