› 3c96

X-ray diffraction
1.9Å resolution

Crystal structure of the flavin-containing monooxygenase phzS from Pseudomonas aeruginosa. Northeast Structural Genomics Consortium target PaR240

Released:
DOI: 10.2210/pdb3c96/pdb
PDB entry 3c96 coloured by chain, front view.
PDB entry 3c96 coloured by chain, side view.
PDB entry 3c96 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Vorobiev SM, Chen Y, Seetharaman J, Wang D, Mao L, Xiao R, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
5-methylphenazine-1-carboxylate + NADH + O(2) = pyocyanine + NAD(+) + CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methylphenazine-1-carboxylate 1-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 44.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HWG9 (Residues: 1-402; Coverage: 100%)
Gene names: PA4217, phzS
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 64.147Å b: 65.261Å c: 88.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.197
Expression system: Escherichia coli

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