› 3caw

X-ray diffraction
1.87Å resolution

Crystal structure of o-succinylbenzoate synthase from Bdellovibrio bacteriovorus liganded with Mg

Released:
DOI: 10.2210/pdb3caw/pdb
PDB entry 3caw coloured by chain, front view.
PDB entry 3caw coloured by chain, side view.
PDB entry 3caw coloured by chain, top view.
Source organism: Bdellovibrio bacteriovorus HD100
Primary publication:
Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero D, Sakai A, Patskovsky Y, Malashkevich VN, Fedorov AA, Bonanno JB, Fedorov EV, Toro R, Agarwal R, Wang C, Ozerova ND, Yew WS, Sauder JM, Swaminathan S, Burley SK, Almo SC, Glasner ME
Proc Natl Acad Sci U S A 111 8535-40 (2014)
PMID: 24872444

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.45 KDa
Source organism: Bdellovibrio bacteriovorus HD100
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6MQC7 (Residues: 1-330; Coverage: 100%)
Gene names: Bd0547, menC
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 53.381Å b: 78.61Å c: 78.462Å
α: 90° β: 91.78° γ: 90°
R-values:
R R work R free
0.218 0.218 0.244
Expression system: Escherichia coli

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Citations

3 review citations

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
2 more

1 mention without citation

Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero et al. (2014)