› 3cb3

X-ray diffraction
2Å resolution

Crystal structure of L-Talarate dehydratase from Polaromonas sp. JS666 complexed with Mg and L-glucarate

Released:
DOI: 10.2210/pdb3cb3/pdb
PDB entry 3cb3 coloured by chain, front view.
PDB entry 3cb3 coloured by chain, side view.
PDB entry 3cb3 coloured by chain, top view.
Source organism: Polaromonas sp. JS666
Entry authors: Fedorov AA, Fedorov EV, Yew WS, Burley SK, Gerlt JA, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Galactarate = (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-171476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 42.99 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12GE3 (Residues: 2-383; Coverage: 100%)
Gene name: Bpro_0435
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand LGT 4 x LGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 195.466Å b: 84.739Å c: 118.247Å
α: 90° β: 126.04° γ: 90°
R-values:
R R work R free
0.21 0.21 0.239
Expression system: Escherichia coli

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